Easier enzymatic determination of guanidinoacetic acid in urine
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چکیده
منابع مشابه
Easier enzymatic determination of guanidinoacetic acid in urine.
(urinary creatinine concentration). Because most of the serum results referred to were below or close to the detection limits of the kits, the urine results would be expected to he negative with the qualitative tests. The NovoClone HCG kit measures intact HCG as well as free 13-chains. The Delfia kit from Pharmacia measures the intact HCG molecule only. This difference might explain the only re...
متن کاملA new enzymic determination of guanidinoacetic acid in urine.
We developed and evaluated a colorimetric method for enzymic determination of guanidinoacetic acid (GAA) in urine. Endogenous urinary urea was first eliminated by urease (EC 3.5.1.5), and the added urease was then removed from the sample by centrifugal ultrafiltration. GAA in the ultrafiltrate was subsequently hydrolyzed by guanidinoacetate amidinohydrolase (EC 3.5.3.2) to glycine and urea. The...
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1THE ACCURATE MEASUREMENT of oxalate concentration in urine presents considerable difficulties. The methods available in the literature are based upon either the direct formation of calcium oxalate precipitate and subsequent oxidimetric titration (1-3) or upon a preliminary ether extraction followed by a titrimetric procedure (4, .5). In a more elaborate method, the extraction with ether is com...
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An ion pair reversed phase HPLC method for the determination of clavulanic acid has been developed. Since clavulanic acid had poor absorption O,mas 201 nm in water) in a UVregion suitable for HPLC detection, the detectability was enhanced by bathochromic shift of )maa due to solvent effect. The shifts of 2ma.., were measured with the solutions containing clavulanic acid, tetrabutylammonium brom...
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We developed a method for the selective determination of 4-aminobenzoic acid by use of 4-aminobenzoate hydroxylase (EC 1.14.13.27) to convert 4-aminobenzoic acid to 4-hydroxyaniline. The subsequent conversion of 4-hydroxyaniline to indophenol dye was quantified colorimetrically. The method is reproducible, and the assay response is linear up to 40 mumol/L. Selectivity exceeding that of the curr...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1991
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/37.3.478